Adam, S., fayyad, D., Darrag, A., Abdelsalam, N. (2021). Comparative evaluation of the cytotoxic effect of different intracanal medicaments. Dental Science Updates, 2(2), 123-133. doi: 10.21608/dsu.2021.46129.1051
Sherouk Hussien Adam; dalia fayyad; Abeer Darrag; Nelly Abdelsalam. "Comparative evaluation of the cytotoxic effect of different intracanal medicaments". Dental Science Updates, 2, 2, 2021, 123-133. doi: 10.21608/dsu.2021.46129.1051
Adam, S., fayyad, D., Darrag, A., Abdelsalam, N. (2021). 'Comparative evaluation of the cytotoxic effect of different intracanal medicaments', Dental Science Updates, 2(2), pp. 123-133. doi: 10.21608/dsu.2021.46129.1051
Adam, S., fayyad, D., Darrag, A., Abdelsalam, N. Comparative evaluation of the cytotoxic effect of different intracanal medicaments. Dental Science Updates, 2021; 2(2): 123-133. doi: 10.21608/dsu.2021.46129.1051
Comparative evaluation of the cytotoxic effect of different intracanal medicaments
2department of endodontics, faculty of dentistry, Suez canal university
3Professor of Endodontics, Faculty of Dentistry, Tanta University
4Department of Endodontics ,Suez Canal University, Faculty of Dentistry, Egypt.
Abstract
ABSTRACT Introduction: intracanal medicaments have an important role in disinfecting of the pulp space and in endodontic regenerative procedures. These medications come in close contact with the periradicular tissue, thus their degradation products must be biocompatible with the periapical tissue. Otherwise, these medicaments will result in intense inflammatory reaction. Aim of the study: The purpose of this in vitro study was to evaluate and compare the cytotoxicity of three intracanal medications; Neem oil, double antibiotic paste (DAP) and calcium hydroxide (Ca(OH)2). Materials and Methods: Immortalized human gingival fibroblast cells were retrieved from the cell bank and then cultured. Three different intracanal medications (Neem oil, DAP, Ca(OH)2) were added to the cultured cells. Cell viability was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and Apoptosis/necrosis assays. Statistical analysis was performed using One-way ANOVA and post-hoc Tukey test. Results: Ca(OH)2 recorded the highest significant cytotoxicity followed by Neem oil, then DAP which recorded the lowest significant cytotoxicity. Cytotoxicity of the three intracanal medicaments used were directly proportional to their concentrations Conclusion: DAP could be better recommended as intracanal medicament in regenerative endodontic procedures because of its biocompatibility compared to the other tested medications.
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