mohamed, A., Tawfik, M., Hegazy, E., ElSharrawy, E. (2024). Histological and Molecular Assessment of Anti-inflammatory Effect of Different Concentrations of Hyaluronic Acid Injected in Inflamed Rabbit’s Tempromandibular Joint. Dental Science Updates, 5(1), 1-11. doi: 10.21608/dsu.2023.238689.1205
anas mohamed; Mona K Tawfik; Enas M. Hegazy; EMAN Abd El-Halim ElSharrawy. "Histological and Molecular Assessment of Anti-inflammatory Effect of Different Concentrations of Hyaluronic Acid Injected in Inflamed Rabbit’s Tempromandibular Joint". Dental Science Updates, 5, 1, 2024, 1-11. doi: 10.21608/dsu.2023.238689.1205
mohamed, A., Tawfik, M., Hegazy, E., ElSharrawy, E. (2024). 'Histological and Molecular Assessment of Anti-inflammatory Effect of Different Concentrations of Hyaluronic Acid Injected in Inflamed Rabbit’s Tempromandibular Joint', Dental Science Updates, 5(1), pp. 1-11. doi: 10.21608/dsu.2023.238689.1205
mohamed, A., Tawfik, M., Hegazy, E., ElSharrawy, E. Histological and Molecular Assessment of Anti-inflammatory Effect of Different Concentrations of Hyaluronic Acid Injected in Inflamed Rabbit’s Tempromandibular Joint. Dental Science Updates, 2024; 5(1): 1-11. doi: 10.21608/dsu.2023.238689.1205
Histological and Molecular Assessment of Anti-inflammatory Effect of Different Concentrations of Hyaluronic Acid Injected in Inflamed Rabbit’s Tempromandibular Joint
2Professor of Pharmacology Faculty of Medicine Suez Canal University
3Department of Oral Biology, Faculty of Dentistry, Suez Canal University
4Professor of Anethesiology, Faculty of Dentistry Suez Canal University
Abstract
ABSTRACT Introduction: Several studies indicated that Hyaluronic acid (HA) has antiinflammatory role through suppressing mediators of inflammation involved in the osteoarthritic disease state. Aim: Investigating the anti-inflammatory effect of different concentrations of hyaluronic acid on experimentally induced inflammatory changes in rabbits temporomandibular joint (TMJ). Material and Methods: Forty male rabbits were prepared and randomly divided into 5 groups each group formed of 8 rabbits as follow: Group (1): 8 animals injected with saline only served a negative control group. Group (2): 8 animals injected with formalin served as positive control. Group (3): 8 animals injected with 0.1ml of formalin once, followed by 2.5mg/0.5ml /week of HA for three weeks. Group (4): formed of 8 animals injected with 0.1ml of formalin once, followed by 5mg/0.5ml /week of HA for three weeks. Group (5): 8 animals injected with 0.1ml of formalin once, followed by 7.5mg/0.5ml /week of HA for three weeks. Eventually blood samples were collected and animals were euthanized. Evaluation in this study included measuring pro inflammatory markers IL-6, IL-1β and CRP by ELISA. Histological evaluation of TMJ tissues to detect changes in the muscles, ligaments, and bones. Results: all animals injected with HA showed a significant reduction in levels of IL-6, IL-1β and CRP in addition to improvement in tissue structure. Animals in G4 and G5 showed significantly better results than G3. Conclusion: there is a statistically significant anti-inflammatory effect of HA on TMJ at different concentrations.