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Amer, M., Hassan, M., Attia, F., Abou El-nour, K., Korraah, A. (2020). XRCC1 immunohistochemical expression in DMBA – induced oral squamous cell carcinoma treated with different thymoquinone preparations. Dental Science Updates, 1(2), 151-159. doi: 10.21608/dsu.2020.25987.1031
Mohamed Hussein Amer; Magda Mohamed Aly Hassan; Fadia Mostafa Attia; Kholoud Mohamed Abou El-nour; Ahmed Mohamed Moheb Eldin Korraah. "XRCC1 immunohistochemical expression in DMBA – induced oral squamous cell carcinoma treated with different thymoquinone preparations". Dental Science Updates, 1, 2, 2020, 151-159. doi: 10.21608/dsu.2020.25987.1031
Amer, M., Hassan, M., Attia, F., Abou El-nour, K., Korraah, A. (2020). 'XRCC1 immunohistochemical expression in DMBA – induced oral squamous cell carcinoma treated with different thymoquinone preparations', Dental Science Updates, 1(2), pp. 151-159. doi: 10.21608/dsu.2020.25987.1031
Amer, M., Hassan, M., Attia, F., Abou El-nour, K., Korraah, A. XRCC1 immunohistochemical expression in DMBA – induced oral squamous cell carcinoma treated with different thymoquinone preparations. Dental Science Updates, 2020; 1(2): 151-159. doi: 10.21608/dsu.2020.25987.1031

XRCC1 immunohistochemical expression in DMBA – induced oral squamous cell carcinoma treated with different thymoquinone preparations

Article 9, Volume 1, Issue 2, September 2020, Page 151-159  XML PDF (764.67 K)
Document Type: Original Article
DOI: 10.21608/dsu.2020.25987.1031
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Authors
Mohamed Hussein Amer email 1; Magda Mohamed Aly Hassan1; Fadia Mostafa Attia2; Kholoud Mohamed Abou El-nour3; Ahmed Mohamed Moheb Eldin Korraah4
1Oral Pathology Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
2Clinical Pathology department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
3Chemistry Department, Faculty of Science, Suez Canal University, Egypt
4Oral Pathology department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
Abstract
The X-ray repair cross-complementing 1 (XRCC1) enzyme plays an important role in the DNA repair pathway. XRCC1 polymorphism increased the risk of human oral squamous cell carcinoma. Loading of thymoquinone on gold nanoparticles as a drug carrier, had revealed a superior anti-cancer effect as a chemotherapeutic agent in DMBA-induced OSCC.
This study aimed to evaluate the expression of DNA repair enzyme X-ray repair cross-complementing 1 (XRCC1) following treatment of induced oral cancer in hamster buccal pouch with thymoquinone (TQ) only and loaded on gold nanoparticles (AuNps).
Sixty male Syrian golden hamsters were divided into 4 groups: Group A: (negative control). The left pouches of the rest of animals were painted with the carcinogen DMBA (3times / week/ 12 weeks), then: Group B: (positive control), Group C: painted and injected intraperitoneally (i.p) with TQ only (3 times/week for 6 and 12 weeks). Group D: painted with TQ loaded on AuNps (3 times/week for 6 and 12 weeks). After euthanization, all pouches were surgically excised, fixed and processed for H&E and XRCC1 immunohistochemical stains.
Groups B, C1, C2, and D1 showed well-differentiated squamous cell carcinoma with low intensity of immune staining. Groups D2 showed remarkable regression of tumors both clinically and histologically with high intensity of IHC staining.
Loading of TQ at low concentration (0.001 mg/kg) on AuNps /12 weeks was a promising chemotherapeutic combination, through enhancing XRCC1 expression to regress the carcinogenesis process. This effect could be due to the anti-oxidant, free-radicle scavenging effect and enhancing apoptosis by TQ.
Keywords
AuNPs; DMBA; Hamster buccal pouch; Squamous cell carcinoma; Thymoquinone and XRCC1
Main Subjects
Oral biology
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