2024-03-29T13:35:12Z
https://dsu.journals.ekb.eg/?_action=export&rf=summon&issue=22511
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Histological study on the effect of the immunosuppressive drugs on the mucous membrane and salivary glands of the treated rats tongues
Loaa
mahmoud
Laila
Ghali
Manar
Selim
Introduction: Tacrolimus is a macrolide calcineurin inhibitor which has been shown to decrease acute and chronic rejection, improved renal function over the longterm post-transplant. Tacrolimus is considered the drug of choice in immunosuppressive therapy for chronic diseases irresponsive to corticosteroids, autoimmune diseases and organ transplantation system. Aim: The aim of the investigation was to study the effect of the immunosuppressive drug Tacrolimus on the lingual mucous membrane and salivary glands of treated rats through hematoxylin and eosin stained sections to detect any microscopic changes in the structure of the mucous membrane and salivary glands of the tongues & Masson’s trichrome stained sections for detection of the integrity of collagen. Material and Methods: Thirty healthy adult male albino rats with body weight ranging from 150 to 180 gm were used in this investigation. The animals were divided into two groups 15 animals each. Group 1: served as control, group 2: animals were treated with the immunosuppressive drug tacrolimus dissolved in distilled water in a daily oral dose of 0.5 mg/kg body weight. At the end of the experiment the rats of different groups were euthanized by cervical dislocation. The tongues were dissected out, fixed, processed, embedded in paraffin, sectioned and stained with hematoxylin and eosin for histological evaluation & Masson’s trichrome stain for demonstration of collagen fibers. Results: The light microscopic examination of group I showed the normal histological features of both surface epithelium and underlying lamina propria, while group II Treated with tacrolimus revealed atrophic and degenerative changes that involved the surface epithelium and lamina propria of both the dorsal and ventral surfaces of the tongue as well as the lingual salivary glands. Conclusion: Tacrolimus administration showed structural alterations in the lingual mucous membrane and the lingual salivary glands which will certainly affect the taste sensation.
Graft rejection
Immunosuppressive agents
Organ transplantation & Tacrolimus
2021
03
01
1
7
https://dsu.journals.ekb.eg/article_152788_3ad4c88859558eac96617022ae016057.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Effect of statins on the periodontal ligament and Alveolar process of induced Diabetic Rats
Yasser
Mohammed
Rasha
Taha
Laila
Ghali
Introduction: Considering the Diabetes mellitus (DM) as one of the oldest conditions characterized in humans, it has been recognized since antiquity and considered as a major health problem at present. There is a need for efficient treatment. Aim: The aim of the present investigation was to find out whether statins can alleviate the well documented side effects of Streptozotocin induced diabetes mellitus on the periodontal ligament of adult male Albino rats. Material and Methods: Sixty adult male albino rats with body weight ranging from180-200gr were used in this investigation. They were divided into the following groups. Group 1: consisted of 20 animals and served as controls. Group 2: consisted of 20 animals, they were subjected to a single intraperitoneal injection of streptozotocin in a dose 60 mg/kg body weightof for induction of diabetes mellitus. Group 3: consisted of 20 animals. They were subjected to induction of diabetes mellitus in the same way as group 2 animals. One week later, the animals were treated with simvastatin in a daily intraperitoneal dose of 5mg/kg body weight. At the end of the experiment the animals were sacrificed by cervical dislocation, their Jaws were dissected out then processed for the histological and transmission electron microscopic examinations. Results: The present work showed that the treatment of diabetic rats with statins resulted in amelioration of the degenerative effect of diabetes on the periodontal ligament when compared with. Changes of diabetes in group 2 and regenerative changes of statins in group 3 compared to the control group 1. Conclusion: Diabetic albino rats treated with statins revealed partial improvements, mostly in the arrangement of the fibers of periodontal ligament.
Alveolar process
diabetes
periodontal ligament
statins
2021
03
01
9
16
https://dsu.journals.ekb.eg/article_152891_0040f318fb4256d8ef0f692f64692209.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Clinical and Radiographic Evaluation of Immediate Implant with Bone Graft (Hypro-oss)
Mona
Abas
Walid
Ghanem
Gihan
El Desouky
Yasser
El Gerby
Introduction: Xenograft play role in osseointegration of immediate dental implant. Aim of the study: to evaluate the efficiency of xenograft (Hypro-oss) in osseointegration of immediate dental implant clinically and radiographically. Patients and methods: This study conducted on 8 patients range from 18-48 years old. 12 immediate dental implants divided into two equal groups; (Control Group): Atraumatic extraction of single rooted tooth or remaining root and immediate placement of dental implant, (Study Group): Atraumatic extraction of single rooted tooth or remaining root and immediate placement of dental implant and sealed with Hypro-Oss bone graft material. Clinical examinations done for all patients prior to surgery include examination of oral and para oral tissues and evaluation the intermaxillary space and the type of occlusion. Radiographic evaluation using CBCT done in the intervals 15, 90, 180 and 270 days postoperatively to measure the bone density of the formed bone between living bone and the implant surface. Result: All the patients showed no signs or symptoms of pain, tenderness, redness or inflammation of soft tissue around the inserted implant site. Normal healing process of the soft tissue and bone around the implant. All patients continued the follow up period. Radiographic evaluation showed significant higher bone density in study group than control group after 15, 90, 180 and 270 days. Conclusion: With the use of Hypro-Oss bone graft, the bone density around the implant showed increase in values.
CBCT
Immediate placement
Xenograft bone grafts
2021
03
01
17
25
https://dsu.journals.ekb.eg/article_152897_5c6628c362f33b760a2911e15fffd98f.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Effect of mesenchymal bone marrow stem cells on the periodontal ligament of induced Diabetic Rats.
Yasser
Mohammed
Rasha
Taha
Laila
Ghali
Introduction: Considering the Diabetes mellitus (DM) as one of the oldest conditions characterized in humans, it has been recognized since antiquity and considered as a major health problem at present. There is a need for efficient treatment. Aim: The aim of the present investigation was to find out whether stem cells can alleviate the well documented side effects of streptozotocin induced diabetes mellitus on the periodontal ligament of adult male albino rats. Material and Methods: Sixty adult male albino rats with body were used in this investigation. They were divided into the following three groups; Group 1: consisted of 20 animals and served as controls; Group 2: consisted of 20 animals, they were subjected to a single intraperitoneal injection of streptozotocin in a dose of 60mg. /kg body weight for induction of diabetes mellitus; Group 3: consisted of 20 animals. They were subjected to induction of diabetes mellitus in the same way as group 2 animals. One week later, the animals were subjected to a single intravenous infusion of mesenchymal bone marrow stem cells. At the end of the experiment one week later the animals were euthanized by cervical dislocation, their jaws dissected out, decalcified then processed for A. histological examination (Hematoxylin and Eosin stain) B. transmission electron microscope. Results: Histological examination in the present work showed that the treatment of diabetic rats with stem cells resulted in amelioration of the degenerative effects of diabetes on the periodontal ligament in group 2 and regenerative changes of stem cells in group 3 compared to the control group 1. Electron microscopic examination of the PDL showed marked regenerative changes in the density of collagen. Conclusion: Diabetic albino rats treated with stem cells revealed partial improvements, mostly in the arrangement of the fibers of periodontal ligament. The ultrastructural features showed normal fibroblasts.
Alveolar process
diabetes
periodontal ligament
stem cells
2021
03
01
27
34
https://dsu.journals.ekb.eg/article_152901_0cc133b75695a5384300415c527c644a.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Clinical Evaluation of Intra-articular Injections of Platelet Rich Plasma Versus Dextrose Prolotherapy for Management of Temporomandibular Joint Internal Derangement
Yasser
Habaka
Eman
Al Sharawy
tamer
bary
Abdelbadia
Abdelmabood
Introduction: TMJ internal derangement is characterized by intra-articular disc displacement resulting in popping or clicking sounds. The displacement of articular disc does not always cause a mechanical obstruction. Those conditions may be with or without pain. Aim: The aim of the study was to evaluate the clinical effect of intra-articular injections of platelet rich plasma for management of TMJ internal derangement through comparative study with dextrose prolotherapy. Patients and Methods: included 87 joints in 45 adult patients complaining pain, clicking in the Temporo-mandibular joint (internal derangement), pre-operative MRI was done for all patients with further clinical, radiographic as well as TMJ examination. Patients were divided into three equal groups randomly. Group I: (study group) composed of 15 patients where 1ml of platelet rich plasma was injected intra-articular (once) Group II: (study group) composed of 15 patients where 1ml of platelet rich plasma was injected intra-articular (twice) with one month interval Group III: (control group) composed of 15 patients where dextrose prolotherapy was injected (4 times) at 2 weeks, 4 weeks and 6 weeks intervals over a total of 12 weeks. Clinical assessment was done including MIO, pain, deviation and clicking. Results: statistically analysis of this study showed that group I, III showed the highest MIO values as well as VAS scores in the 3,6 and 12 months, however group II showed the lowest MIO in the same follow up period. Conclusions: PRP and Prolotherapy are successful techniques for treatment of symptoms related to internal derangement of TMJ.
internal derangement
TMD’s
Prolotherapy
PRP injection
2021
03
01
35
46
https://dsu.journals.ekb.eg/article_152912_a05ce88ddf066a8b09b92cd9ebcdfaee.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Comparative study between Sodium hyaluronic acid and dextrose prolotherapy in management of Temporo-mandibular joint internal derangement.
Yasser
Habaka
Eman
Al Sharawy
tamer
bary
abdelbadia
abdelmabood
Introduction: Internal derangements of TMJ were nonsurgical treated in initial stages with medications, physiotherapy and occlusal splints. Intra articular injection of corticosteroids alone or after arthrocentesis provides long-term palliative effects on clinical signs and subjective symptoms of TMJ pain. Aim: The study was done to compare between hyaluronic acid and dextrose prolotherapy in management of temporomandibular joint internal derangement. Patients and methods: The present study included 30 adult patients suffering from internal derangement of Temporo-mandibular joint were selected from outpatient clinic, oral and maxillofacial surgery department in faculty of dentistry, Suez Canal University as well as October 6 university. Group I: Composed of 15 patients (10 females, 5 males) where hyaluronic acid was injected intra-articular. Group II: Composed of 15 patients (9 females, 6 males) where dextrose prolotherapy was injected (4 times) at 2 weeks, 4 weeks and 6 weeks intervals over a total of 12 weeks. Results: No difference between Group I and II; both showed the highest mean MIO values. Group I and Group II; both showed lower mean of VAS scores after 12 months compared to pre-operative measurement. After 6 months, Group II showed the highest prevalence of deviation followed by Group I. Conclusion: Sodium hyaluronic acid and Prolotherapy is a successful technique to improve maximum inter-incisal opening as well as assisted interincisal opening and improved higher significant changes in pain intensity.
TMJ
internal derangement
PRP
Hyaluronic acid
Arthrocentesis
Dextrose prolotherapy
2021
03
01
47
54
https://dsu.journals.ekb.eg/article_152923_e4f51629788d50e124779a5a301890a2.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Effect of Single Session of Low-Level Laser Therapy on Pain, Swelling, and Trismus after Surgical Extraction of Impacted Lower Third Molar : A Clinical Study
Mina
Girgis
Eman
Elsharrawy
Mohamed
Hassan
Introduction: Low-level laser therapy (LLLT) can promote and accelerate the tissue regeneration as it causes the early healing of extraction sockets but the accurate mechanism is unknown. Aim: The study aimed to evaluate the effect of LLLT which was applied in a single session immediately after odontectomy of impacted lower wisdom tooth on pain, swelling, and trismus. Patient and Methods: This study was done on 24 healthy patients who required surgical extraction of impacted lower third molar. The patients were randomly selected and randomly divided into two equal groups, each consisted of 12 patients for group, group I received LLLT immediately after surgical extraction of impacted lower third molar, group II was subjected to routine surgical extraction of impacted lower third molar without application of LLLT. LLLT was applied at ten points: four intra-oral beside to the extracted socket and six extra-oral along the masseter muscle. Laser energy was applied at 100mW for a total of 150 sec, 15 sec for each point. Results: The results showed the effect of a single session of LLLT in control of postoperative pain on the day of surgery and reduction of swelling and trismus after surgical removal of impacted lower third molars (p < 0.05). Conclusion: LLLT could represent a viable tool to control pain in patients who are contraindicated to the medications.
Laser
LLLT
Pain
swelling
Trismus
2021
03
01
55
60
https://dsu.journals.ekb.eg/article_152925_0b0822451f8ade9d7a6fae5494d2c073.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Synergistic Therapeutic Effect of Honokiol and 5-Fluorouracil on the Induced-Tongue Cancer in Rats.
Sara
Swidan
Magda
Hassan
Merhan
El mansy
Introduction: 5-Fluorouracil (5-FU) is a common chemotherapeutic drug for treatment of oral squamous cell carcinoma. However, its toxicity to normal tissues has limited its role as an effective cancer therapy. Aim: This research aimed to investigate the effect of combining 5-FU with honokiol (HNK) on enhancing the anticancer activity of 5-FU without increasing its toxicity. Material and methods: HNK was used in combination with 5-FU for treatment of tongue carcinoma induced chemically by 4-nitroquinoline 1 oxide (4-NQO) in albino rats. Rats were divided into five groups (10 animals each) including control, 4-NQO, 5-FU, HNK, and combined 5-FU with HNK. Serial sections of the tongues from all animals were examined microscopically and real-time PCR quantification of P53 gene expression was also assessed. Results: The results showed that treatment with both 5-FU + HNK had significantly regressed the number and size of malignant lesions seen in the carcinogen-only group. Data analysis revealed a statistically significant decrease in the expression levels of mutant P53 in the combined treatment group compared to control groups. Conclusion: This study reported that HNK and 5-FU had a synergistic cytotoxic effect on this model, without notable intensification of the side effects associated with 5-FU treatment.
Introduction: 5-Fluorouracil (5-FU) is a common chemotherapeutic drug for treatment of oral squamous cell carcinoma. However, its toxicity to normal tissues has limited its role as an effective cancer therapy. Aim: This research aimed to investigate
the effect of combining 5-FU with honokiol (HNK) on enhancing the anticancer activity of 5-FU without increasing its toxicity. Material and methods: HNK was used in combination with 5-FU for treatment of tongue carcinoma induced chemically by
4-nitroquinoline 1 oxide (4-NQO) in albino rats. Rats were divided into five groups (10 animals each) including control, 4-NQO, 5-FU, HNK, and combined 5-FU with HNK. Serial sections of the tongues from all animals were examined microscopically and
real-time PCR quantification of P53 gene expression was also assessed. Results: The results showed that treatment with both 5-FU + HNK had significantly regressed the number and size of malignant lesions seen in the carcinogen-only group. Data analysis
revealed a statistically significant decrease in the expression levels of mutant P53 in the combined treatment group compared to control groups. Conclusion: This study reported that HNK and 5-FU had a synergistic cytotoxic effect on this model, without
notable intensification of the side effects associated with 5-FU treatment.
Fluorouracil
Honokiol
Tongue
OSCC
p53
PCR
2021
03
01
61
68
https://dsu.journals.ekb.eg/article_152927_7a0eeec059366b25f43354c1978b9251.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Evaluation of Some Salivary Parameters in a Group of Children Suffering From Rampant Caries in Ismailia City
Eman
Aboelliel
Ghada
Elbaz
Nagwan
Sabek
Asmaa
ABO ELSOUD
Introduction: Rampant caries (RC) is a severe form of dental decay with multifactorial origin. RC poses a challenging management problem for dentists and patients. Aim: the aim of the study was to evaluate and compare between some salivary parameters: pH, buffering capacity, total protein, flow rate and zinc concentration in a group of children suffering from rampant caries and others with normal caries activity in Ismailia city. Material and methods: sixty apparently healthy children aged from 3-5 years of both sexes. According to dmfs index the selected children were divided into three groups. Saliva samples were examined for salivary pH changes, measured by pH meter, Buffering capacity by titratable acidity, Total protein by colorimetric method, Flow rate by drooling method and Zinc concentration using an atomic absorption spectrophotometer. Results: there was no statistically significant difference between mean salivary pH values in three groups. Salivary buffering capacity results showed, group I had a statistically significantly highest mean buffering capacity, There was no statistically significant difference between group II and group III, salivary total proteins showed no statically significant difference between group I and group II; both showed the statistically significant highest mean total protein, but group III showed the statistically significant lowest mean salivary total protein. There was no statistically significant difference between the three groups in salivary flow rate and Zn concentration. Conclusion: salivary pH, flow rate and Zn conc. can’t be used as an indicator for occurrence of rampant caries but salivary total protein and salivary buffering capacity may be used.
Introduction: Rampant caries (RC) is a severe form of dental decay with multifactorial
origin. RC poses a challenging management problem for dentists and
patients. Aim: the aim of the study was to evaluate and compare between some salivary
parameters: pH, buffering capacity, total protein, flow rate and zinc concentration in a
group of children suffering from rampant caries and others with normal caries activity
in Ismailia city. Material and methods: sixty apparently healthy children aged from
3-5 years of both sexes. According to dmfs index the selected children were divided
into three groups. Saliva samples were examined for salivary pH changes, measured
by pH meter, Buffering capacity by titratable acidity, Total protein by colorimetric
method, Flow rate by drooling method and Zinc concentration using an atomic
absorption spectrophotometer. Results: there was no statistically significant difference
between mean salivary pH values in three groups. Salivary buffering capacity results
showed, group I had a statistically significantly highest mean buffering capacity, There
was no statistically significant difference between group II and group III, salivary
total proteins showed no statically significant difference between group I and group < /span>
II; both showed the statistically significant highest mean total protein, but group III
showed the statistically significant lowest mean salivary total protein. There was no
statistically significant difference between the three groups in salivary flow rate and
Zn concentration. Conclusion: salivary pH, flow rate and Zn conc. can’t be used as
an indicator for occurrence of rampant caries but salivary total protein and salivary
buffering capacity may be used.
Rampant caries (RC)
salivary power of hydrogen(pH)
salivary flow rate
salivary total protein
salivary buffering capacity
2021
03
01
69
75
https://dsu.journals.ekb.eg/article_152929_7ad9bf78b8f0d2b52857963c845fcc25.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Combined Photothermal and Nanochemotherapy in Treatment of Induced Oral Squamous Cell Carcinoma in Hamsters
Randa
El-sherbiny
Magda
Hassan
Wafaa
El-Hossary
Mona
Shata
Introduction: Oral squamous cell carcinoma (OSCC) shows unsatisfied survival rate which remains not changed over the last years. Thymoquinone (TQ) is a phytochemical, and many studies proved its promising therapeutic effect against OSCC. Gold nanorod (GNR) is a photothermal agent, has an efficient near-infrared heat conversion and induces ablation of solid tumors. The combination of photothermal therapy (PTT) and chemotherapy can enhance synergistic effects that markedly exceed the sum of individual treatments alone. Aim: this study aimed to evaluate the effect of combined photothermal and nano chemotherapy in treatment of induced oral squamous cell carcinoma in hamsters. Material and Methods: In the present study, the combined drug GNR/NTQ loaded on (poly) (lactic-co-glycolic acid) (PLGA) was prepared. The targeting agent, folate or folic acid (FA), was conjugated to the prepared nanoparticles. The therapeutic activity of the drug against chemically-induced OSCC in hamster buccal pouches was evaluated by clinical observations, tumor volume analysis, histopathological alterations and blood analysis. Results: The combined drug (GNR/NTQ/PLGA/FA/laser) had the highest therapeutic activity compared to individual treatments. It significantly ablated and regressed tumor size in chemically-induced OSCC in the hamster model as compared to the other preparations; GNR/NTQ/PLGA/FA without laser, GNR/ PLGA/FA/ laser and NTQ/PLGA/FA/laser. Conclusion: Compared to the single PTT or nanochemotherapy, the combined PTT/nanochemotherapy with laser provides better therapeutic effects against OSCC
folate
Gold nanoparticles
Oral squamous cell carcinoma
Thymoquinone
2021
03
01
77
88
https://dsu.journals.ekb.eg/article_152933_b21fcdeb7483492aca524889946c2b93.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
The Effect of Nano-hybrid Composite Resin Denture Teeth on Mandibular Residual Ridge
Maha
Moussa
Moataz
Elmahdy
Sayed
El masry
Hala
Ali
Introduction:Progressive ridge resorption is one of the main causes of loss of stability and retention of mandibular complete dentures with subsequent loss of masticatory efficiency and patient discomfort. Aim: This study was conducted to evaluate radiographically the effect of nano-hybrid composite resin teeth on mandibular residual ridge using cone beam computerized tomography (CBCT). A comparative study. Materials and Methods: Twelve completely edentulous male patients were randomly divided into two groups according to the artificial tooth material used in complete denture construction. One group received complete dentures with nano-hybrid composite resin teeth and the other group received complete dentures with cross-linked acrylic resin teeth. CBCT was taken at the delivery, 6 months and 12 months follow -up appointments. The evaluation was done for height and width changes of the mandibular residual alveolar ridge at different anatomical sites either in the anterior or posterior part of the mandible. Results: Analysis was done by using two-tailed t-test revealed that increase in amount of bone loss in both groups which is in favor to patients wearing dentures with nano-hybrid composite resin teeth, with no statistically significant difference between the two groups. Conclusion: Despite of introduction of new denture tooth material as nano-hybrid composite resin teeth, the cross-linked acrylic resin teeth still considered the gold standard denture teeth regarding the degree of occlusal force transmission and preservation of residual alveolar ridge.
Cone beam computed tomography
nanocomposite denture teeth
ridge resorption
2021
03
01
89
95
https://dsu.journals.ekb.eg/article_152934_50021832fd95982569614bb0a44041ac.pdf
Dental Science Updates
2636-3836
2636-3836
2021
2
1
Effect of Different Locator heights on peri-implant bone changes in Mandibular implant retained Overdenture
Helmy
Abdelhafiz
Moataz
Elmahdy
Sayed
Elmasry
Hala
Abd El-Hamid
Introduction: The implant supported overdentures offered better chewing and masticatory functions. Locator attachment has more advantages than other attachment types. Aim: The aim of the study was to compare radio-graphically the effect of implant locator’s height on peri-implant bone height and density in mandibular over-denture. Patients and method: Twelve completely edentulous male patients had received conventional complete maxillary dentures and mandibular overdenture that retained by two implants that were inserted in the canine region by the help of CAD/CAM based surgical guides. The patients were divided randomly into two groups. Group I: the patients were received locator abutment with 1mm in height. Group II: the patients were received locator abutment with 3mm in height, a series of cone beam radiographs were taken at 0, 6, 12 and 18 months starting from the first day of lower over denture installation, peri-implant bone changes regarding height and density were estimated. Results: It was found that the mean marginal bone loss measurements were relatively higher for group I than group II with non-statistical significant difference. The average difference in bone density measurements around the implants for both groups showing initial increase followed by marked decrease through the follow up periods. Conclusion: locator height has an effect on peri-implant bone height, bone density.
bone density
Implants
marginal bone
Locator height
2021
03
01
97
104
https://dsu.journals.ekb.eg/article_152935_12ccb7ba8310397a5964ede8c7f67294.pdf