ORIGINAL_ARTICLE
Histomorphometric Evaluation of the Healing Process of Bony Defects Filled with Milled Teeth Versus Beta Tri-Calcium Phosphate Bone Graft Materials in Rats.
Objectives: the aim of the study was the evaluation of healing in bony defects comparing milled teeth versus beta tricalcium phosphate (B-TCP) as bone graft materials. Materials and methods: Forty-five male albino rats, were divided into three groups each group included 15 rats; one control and two experimental groups. Under general anesthesia, 1.5 cm incision was created to expose the cranial bone. Then two bone defects (each one with a diameter of 3 millimeter) were created one in each parital sides. In Group I (control group): the two bony defects (30 bony defects) were left to heal without any intervention. While, in Group II & III the bony defects were completely filled with (B-TCP) and milled teeth, respectively. At the 7th, 15th, and 30 days postoperatively five rates were randomly selected from each group to be sacrificed. Results: histomorophometric and histological results showed a higher level of bone regeneration in the two experimental groups than that of the control group all over the study period, moreover there was a significant increase in the amount of (newly formed bone) in the milled teeth than in the (B-TCP) group. The remaining particles of the two graft materials was significantly less in the milled teeth group than in the (B-TCP) group during the whole study time. Conclusion: Bone activity, formation and maturity were ahead in tooth ash filled calvarial bony defects.
https://dsu.journals.ekb.eg/article_110350_fc0e77a8df2d98e10cfd84de3c75a26c.pdf
2020-09-01
75
83
10.21608/dsu.2020.13793.1001
Bone graft material
Beta tricalcium phosphate
histomorphometry
Tooth ash
Ghada
Hassan
ghhassan90@gmail.com
1
PhD candidate, oral biology dept. suez canal university, egypt working at zagazig university hospitals , zagazig university, zagazig. egypt
LEAD_AUTHOR
Heba
Elsaied
hebaameen28@gmail.com
2
assistant professor oral biology dept., dental college, suez canal university
AUTHOR
Laila
Ghali
laila.sadek@su.edu.eg
3
professor oral biology, dental college, cairo university dean of dental college sini university, qantara branch, egypt
AUTHOR
ORIGINAL_ARTICLE
Transmission Electron Microscopic Examination of the Healing Power Effect of Milled Teeth Versus Beta- Tri Calcium Phosphate Grafted in Calvarial Bone Defect in Rats.
Objectives: to evaluate the effect of milled teeth versus beta tricalcium phosphate bone graft material on new bone formation at the cell level using transmission electron microscope Materials and methods: Forty-five adult male albino rats, were divided into three main groups (n=15); control and two experimental groups. Under general anesthesia, 1.5 cm incision was done exposing the cranial bone. Then two noncritical size bone defects were created one in each parital sides of the rat calvarial bone. In Group I (control group): the bilateral calvarial bony defects were left to heal spontaneously. In Group II the bilateral calvarial bony defects completely filled with β-TCP. In Group III: the bilateral calvarial bony defects completely filled with tooth ash. Five rats from each group were sacrificed after 7, 15, and 30 days postoperatively. Results: tooth ash group: showed more new bone formation. Different types of cells appeared more active: multiple lymphocytes, fibroblast, osteoprogenitor cells, osteoblasts. Multinucleated osteoclasts with prominent vacuolated cytoplasm and ruffled border in Howship’s lacunae were clearly seen. At day 30, disappearing of the tooth ash and infiltration of different types of cells; osteoprogenitor cells, bone lining cells, and osteoblasts while β-TCP particles were still seen in the sections. Conclusion: Bone activity, formation and maturity were ahead in tooth ash filled calvarial bony defects. Tooth ash had a role in the stimulation of osteoblastic activity in matrix formation and its mineralization.
https://dsu.journals.ekb.eg/article_110351_120dd4e6555d4884152f82995dcd65c0.pdf
2020-09-01
85
94
10.21608/dsu.2020.15695.1007
Bone graft material
Beta tricalcium phosphate
Milled teeth
Transmission electron microscope
Ghada
Hassan
ghhassan90@gmail.com
1
PhD candidate, oral biology dept. suez canal university, egypt working at zagazig university hospitals , zagazig university, zagazig. egypt
LEAD_AUTHOR
Heba
Elsaied
hebaameen28@gmail.com
2
assistant professor oral biology dept., dental college, suez canal university
AUTHOR
Laila
Ghali
laila.sadek@su.edu.eg
3
professor oral biology, dental college, cairo university dean of dental college sini university, qantara branch, egypt
AUTHOR
ORIGINAL_ARTICLE
Two Different Techniques for Alveolar Ridge Expansion
Abstract: Aim of the study: To evaluate the efficacy of two different techniques for maxillary alveolar ridge expansion, the osteotome and screwexpander. Patients and methods: This study was carried out on 16 healthy male patients received 16 implants. Average age 40 years. Each patient has partial edentulous maxilla with bone width not less than 4 mm and height not less than 10 mm. The patients were divided into two equal groups. In group I, implant site was prepared by osteotomes of increasing diameter to reach the planned osteotomy width, group II, implant site was prepared by expansion screws of increasing diameter to reach the planned osteotomy width. Clinical evaluation was done 24 hours postoperatively for edema, wound dehiscence, pain and signs of infection and regular checkups were done weekly during the first month. Radiographic evaluation using CBCT was done after 3 and 9 months postoperatively to measure bone density around inserted implants and vertical bone loss. Implant stability was measured by using Osstell ISQ device for all the implants of the two groups after 6 months (before loading) and after 9 months. Results: Radiographic evaluation showed significant higher bone density and lesser vertical bone loss around the inserted implants in group I than in group II after 3 and 9 months. Also, implant stability measurements at six and nine months showed highly significant values in group I more than in group II. Conclusion: Maxillary alveolar ridge expansion with osteotome is superior to screwexpander regarding to bone density and osseointegration.
https://dsu.journals.ekb.eg/article_110352_116154c3493a917806a319fcc1159c5a.pdf
2020-09-01
95
102
10.21608/dsu.2020.16421.1008
Implant
maxillary ridge expansion
osteotome
screwexpander
Ahmed
Ghazy
dr.ah.ghazy@gmail.com
1
Oral and maxillofacial surgery department, Faculty of dentistry, Suez Canal University.
LEAD_AUTHOR
Walid
Ghanem
walid_dent@yahoo.com
2
oral and maxillofacial surgery department,faculty of dentistry,suez canal university
AUTHOR
ORIGINAL_ARTICLE
Shear Bond Strength of a Contemporary Dental Adhesive System to Two Types of Zirconia
Aim: to evaluate the shear bond strength of an adhesive system (Single bond universal adhesive+ RelyX Ultimate cement) and Panavia F2.0 to two types of zirconia (conventional ZI zirconia and translucent TZI zirconia) after thermocycling. Materials & Methods: 10 square shaped large samples from Incoris ZI blocks , and 10 from Incoris TZI blocks were prepared with larger dimensions (12x12x2mm). Another 10 square shaped small samples from each type of zirconia blocks were with small dimensions (6x6x2mm). The surfaces of the large and small samples were air abraded with 110 µm AL2O3 particles, then each small sample was bonded to the large sample using two types of cements (RelyX Ultimate cement in combination with Single bond universal adhesive and Panavia F2.0 cement) under 3Kg load and light cured. After bonding, all specimens were thermocycled for 5000 cycles. The cement/zirconia interface for each subgroup was detected under scanning electron microscope and the shear bond strength was tested using a computerized universal testing machine. Results: No significant difference between shear bond strength of all subgroups tested. Conclusion: The shear bond strength of RelyX Ultimate+ Single bond universal adhesive to two types of zirconia was comparable to Panavia F2.0 , and the shear bond strength of translucent zirconia bonded with two types of cements was comparable to conventional zirconia. Keywords: Panavia F2.0, RelyX Ultimate, Sandblasting , Single Bond Universal Adhesive, Zirconia.
https://dsu.journals.ekb.eg/article_110353_44db34210f1ed313b5cd4fa124a6e263.pdf
2020-09-01
103
112
10.21608/dsu.2020.16570.1012
Panavia F2.0
RelyX Ultimate
Sandblasting
single bond universal adhesive
Zirconia
Heba
Eldawy
heba_3776@hotmail.com
1
fixed prosthodontic,faculty of dentistry,suez canal university
LEAD_AUTHOR
mosaad
Elgabarouny
dr.mosaad@yahoo.com
2
professor in crown and bridge department, faculty of dentistry, suez canal university
AUTHOR
Diaa
Saad
dsaad7@hotmail.com
3
Lecturer in Crown and Bridge Department- Faculty of dentistry- Suez Canal University
AUTHOR
Amr
Shebl
amrsheblks@gmail.com
4
Lecturer in Crown and Bridge Department - Faculty of dentistry - Suez Canal University
AUTHOR
ORIGINAL_ARTICLE
comparative study between two types of bone graft in dental implant
ABSTRACT: Introduction osseointegration around dental implant is the key word of implant stability and bone grafts have a big share for enhancing osseointegration . Aim of the study: To evaluate the efficacy of two different types of bone graft, the (OsteonII) and (Biogen). Patients and methods: This study was carried out on fifteen healthy male patientswith averageage from 20 to 40 years old.. 15 immediate dental implants were dividedinto (group1)immediate placement of 6 dental implant into fresh extracted bony socket with bone graft OsteonII,(group 2) immediate placement of 6 dental implant with bone graft BioGen and in, (group 3)immediate placement of 3dental implant into fresh extracted bony socket without bone graft.Clinical examination was donefor all patients befor surgery. Radiographic evaluation using CBCT was done after 2weeks and 3, 6 months postoperatively to measure bone density around inserted implants and vertical bone loss. Results: Radiographic evaluation showed significant higher bone density and lesser vertical bone loss around the inserted implants in groupII than groupI after two weeks, 6 and 9 months. Also, implant stability measurements at six and nine months showed higher values in group II than in group I. 4 / 21 Conclusion: immediate implant placement can be done successfully using either OsteonII or BioGen bone grafts but, BioGenare superior with using immediate dental implant than OsteonII regarding bone density and implant stability around implant. Key words:Alloplastic bone grafts-Immidiate placement-CBCT–Implant stability.
https://dsu.journals.ekb.eg/article_110354_7bbb31ad57cfb779a4acabc2f3fad5d9.pdf
2020-09-01
113
120
10.21608/dsu.2020.18382.1016
bone
Grafts
Immediate
placement
CBCT
stability
Amr
Kamel
amr.abdelnasser@hotmail.com
1
Faculty of dentistry, misr international university,cairo,egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
Molecular evaluation of the chemotherapeutic effect of thymoquinone loaded on gold nanoparticles through expression of DNA repair enzymes in induced oral squamous cell carcinoma
Different types of DNA damages occur during the induced oral carcinogenesis, which can be eliminated through several DNA repair pathways. XRCC1 and ERCC1 are the main repair enzymes involved in repair of oral squamous cell carcinoma. A combination of thymoquinone with gold nanoparticles as a novel chemotherapeutic modality is the aim of the present work against chemically induced SCC in the classic model of HBP/DMBA carcinogenesis. One hundred male Syrian golden hamsters were divided into: Group A: Ten animals (negative control), group B: Ten animals (positive control) painted with DMBA only 3times / week/ 12weeks. The rest of animals were painted with DMBA (3times / week/ 12weeks) then painted and injected intraperitoneal with TQ only, 0.01mg/kg TQ-GNps, 0.001mg/kg TQ-GNps or GNps only for 6- and 12- weeks, intervals. By end of the experiment, both pouches from all groups were surgically excised, fresh samples from each pouch were processed for RT-PCR technique. The rest of the pouches were fixed and processed for H&E evaluation. Loading of thymoquinone on gold nanoparticles was promising chemotherapeutic combination, through regression of well-differentiated SCC (positive control) to dysplasia and enhanced expression of the studied DNA repair enzymes compared to either thymoquinone or gold nanoparticles groups.
https://dsu.journals.ekb.eg/article_110355_8f7d1626aacc03ed0dee44224a14953b.pdf
2020-09-01
121
131
10.21608/dsu.2020.19585.1015
DMBA
ERCC1
Gold nanoparticles
Hamster buccal pouch
RT-PCR
Thymoquinone and XRCC1
Mohamed
Amer
firstdentist82@gmail.com
1
Oral Pathology Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
LEAD_AUTHOR
Magda
Hassan
ma17_10@yahoo.com
2
Oral Pathology Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
AUTHOR
Fadia
Attia
fadiamostafa@gmail.com
3
Clinical Pathology department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
AUTHOR
Kholoud
Abou El-nour
kabolnoor@yahoo.com
4
Chemistry Department, Faculty of Science, Suez Canal University, Egypt
AUTHOR
Ahmed
Korraah
ahmedmohebk@hotmail.com
5
Oral Pathology department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
AUTHOR
ORIGINAL_ARTICLE
Clinical and Radiodensitometric Evaluation of Low Energy Laser in Management of Periodontal Condition in Smoker Patients
The present investigation was conducted to evaluate clinically and radiodensitometrically the role of Low Energy Laser in the management of periodontal condition in Smoker Patients. The study was performed on twenty four medically free patients having chronic periodontitis. Radiographic bone density evaluation and clinical periodontal parameters were recorded after the initial phase of plaque control then, the patients were instructed to perform oral hygiene measures. The clinical parameters which were included are; plaque index, gingival index, probing depth and clinical attachment loss. The patients were divided into 2 main groups, the first group received Laser therapy, while the second group served as control group. At baseline, three and six months, bone density and alveolar bone level were radiographically recorded using CBCT. The statistical analysis of the collected clinical and radiographic data revealed an overall clinical as well as Radiometric and Densitometric measurements improvement by time for the laser group in comparison to the control group at baseline, three and six months.
https://dsu.journals.ekb.eg/article_110356_dfd5b8242a6d4751eb26bd3df606a27f.pdf
2020-09-01
133
140
10.21608/dsu.2020.21528.1018
Low Energy Laser
Periodontal condition
Smoker Patients
Hisham
El Sheikh
hisham.elsheikh@bue.edu.eg
1
Oral Radiology Faculty of dentistry the British university in Egypt Cairo
LEAD_AUTHOR
Wael
Amer
wael_amer@dent.suez.edu.eg
2
Dean of faculty of dentistry Suez canal university ,Oral radiology department
AUTHOR
Mouchira
El Din
tarek.abbas@bue.edu.eg
3
Professor of oral medicine and periodontology and oral radiology faculty of dentistry Cairo university
AUTHOR
Ahmed
El Rawdy
ahmadelrawdy@yahoo.com
4
Associate professor in oral radiology department faculty of dentistry in Suez canal university
AUTHOR
ORIGINAL_ARTICLE
Evaluation of Microleakage and Microtensile Bond Strength of Two Bulk Fill Composites in Primary Teeth after Caries Removal by Chemomechanical Technique
Aim: Evaluation of microleakage and microtensile bond strength of two bulk fill composites (X-tra fill packable and X-tra base flowable bulk fill composites) in primary teeth after chemomechanical caries removal. Material and methods: Forty extracted human primary posterior teeth with caries not involving pulp exposure were assigned after caries removal by Papacarié into two groups according to restorative material used, each containing 20 teeth. Group 1: was restored with X-tra fill. Group 2: was restored with X-tra base. In two groups Futurabond dual-curing self-etch bond was used to bond restorations to the dentin. Both groups were subdivided into two subgroups: Sub-group A: 10 teeth for microleakage test. Sub-group B: 10 teeth for microtensile bond strength test. For microleakage test: After thermocycling; teeth were covered with varnish then immersed in 2% methylene blue for 24 hours, thereafter the dye penetration was assessed with measured in micrometer using MA100 fully configured Halogen inverted scope. For microtensile test: The samples were sectioned longitudinally to the long axis of the tooth to obtain sticks of standardized cross sectional area. These sticks were stressed to failure under tensile force in a universal testing machine. The µTBs for each specimen was calculated in megapascal. Results: X-tra Fill showed lower insignificant mean microleakage compared to X-tra Base at P value =0.3. X-tra Fill showed higher insignificant mean µTBs (MPa) compared to X-tra Base at P value =0.097. Conclusion: Both bulk fill composites have performed approximately similar regarding microleakage and µTBs after using Papacarie in caries removal.
https://dsu.journals.ekb.eg/article_110357_2429144a03a37dd42e0934df6e6b1d55.pdf
2020-09-01
141
150
10.21608/dsu.2020.22350.1020
Bulk fill composite
Caries removal
Chemo-mechanical
Papacarie
Fatma
Elhendawi
hesham.mohammed86@yahoo.com
1
B.D.S Mansoura University (2010)
General dental practitioner in the Ministry of Health
LEAD_AUTHOR
ORIGINAL_ARTICLE
XRCC1 immunohistochemical expression in DMBA – induced oral squamous cell carcinoma treated with different thymoquinone preparations
The X-ray repair cross-complementing 1 (XRCC1) enzyme plays an important role in the DNA repair pathway. XRCC1 polymorphism increased the risk of human oral squamous cell carcinoma. Loading of thymoquinone on gold nanoparticles as a drug carrier, had revealed a superior anti-cancer effect as a chemotherapeutic agent in DMBA-induced OSCC. This study aimed to evaluate the expression of DNA repair enzyme X-ray repair cross-complementing 1 (XRCC1) following treatment of induced oral cancer in hamster buccal pouch with thymoquinone (TQ) only and loaded on gold nanoparticles (AuNps). Sixty male Syrian golden hamsters were divided into 4 groups: Group A: (negative control). The left pouches of the rest of animals were painted with the carcinogen DMBA (3times / week/ 12 weeks), then: Group B: (positive control), Group C: painted and injected intraperitoneally (i.p) with TQ only (3 times/week for 6 and 12 weeks). Group D: painted with TQ loaded on AuNps (3 times/week for 6 and 12 weeks). After euthanization, all pouches were surgically excised, fixed and processed for H&E and XRCC1 immunohistochemical stains. Groups B, C1, C2, and D1 showed well-differentiated squamous cell carcinoma with low intensity of immune staining. Groups D2 showed remarkable regression of tumors both clinically and histologically with high intensity of IHC staining. Loading of TQ at low concentration (0.001 mg/kg) on AuNps /12 weeks was a promising chemotherapeutic combination, through enhancing XRCC1 expression to regress the carcinogenesis process. This effect could be due to the anti-oxidant, free-radicle scavenging effect and enhancing apoptosis by TQ.
https://dsu.journals.ekb.eg/article_110358_6f74be45fb09434aa7c3271c13d189af.pdf
2020-09-01
151
159
10.21608/dsu.2020.25987.1031
AuNPs
DMBA
Hamster buccal pouch
Squamous cell carcinoma
Thymoquinone and XRCC1
Mohamed
Amer
firstdentist82@gmail.com
1
Oral Pathology Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
LEAD_AUTHOR
Magda
Hassan
ma17_10@yahoo.com
2
Oral Pathology Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
AUTHOR
Fadia
Attia
fadiamostafa@gmail.com
3
Clinical Pathology department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
AUTHOR
Kholoud
Abou El-nour
kabolnoor@yahoo.com
4
Chemistry Department, Faculty of Science, Suez Canal University, Egypt
AUTHOR
Ahmed
Korraah
ahmedmohebk@hotmail.com
5
Oral Pathology department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
AUTHOR
ORIGINAL_ARTICLE
Synergistic Effect of Resveratrol with 5-Fluorouracil in Chemotherapy of Induced Oral Squamous Cell Carcinoma
Aim: To evaluate the anticancer effect of resveratrol alone or combined with 5-Fu on experimentally- induced tongue cancer in rats. Methodology: Forty male albino rats were divided into five groups: Group I: untreated (negative control), group II: positive-control, receiving 50 ppm 4-NQO in drinking water, group III: received i.p injection of (5-FU), group IV: oral-administration of resveratrol (RV), and group V: receiving both RV and 5-FU. Before euthanasia, blood samples were withdrawn to evaluate serum level of ALT, AST and creatinine. After euthanasia, the tongues were surgically excised, and processed for H&E, and mutant p53 IHC. The mean of positive cells/group were statistically analyzed. Results: Group I showed normal tongue mucosa with negative immune expression of mutant p53 cells. Group II revealed malignant ulcers in a form of well/moderately differentiated SCC, and the highest mean of mutant p53 immuno-expression. Group III showed mild dysplasia of the tongue epithelium with a significant regression of the mean number of mutant p53 positive-cells. Group IV expressed moderate dysplasia, with a significantly high mean of mutant p53. Group V showed mild dysplasia with comparable mean of mutated p53 positive cells. Regarding toxicity, group II showed the maximum elevation of ALT, AST, and serum creatinine. These levels were decreased significantly in the treated groups, but high compared to Group I. Conclusion: Resveratrol has a promising therapeutic effect against 4NQO- induced tongue cancer as 5-FU alone. Also, it diminished the cytotoxic effect of 5-FU and enhanced normal histologic-appearance of tongue mucosa when used in combination.
https://dsu.journals.ekb.eg/article_110359_b43738ec9d1596798f818de7b80cf4f3.pdf
2020-09-01
161
174
10.21608/dsu.2020.30956.1038
Chemotherapy
Fluorouracil
IHC
Oral squamous cell carcinoma
p53
Resveratrol
Dina
Eloraby
dinaelorabyy@gmail.com
1
Oral pathology department, faculty of dentistry, Misr international university, Cairo, Egypt
LEAD_AUTHOR
Magda
Hassan
magdahassan@gmail.com
2
B.D.S., PhD, Professor of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
AUTHOR
Marwa
El.shafei
marwaelshafei@gmail.com
3
B.D.S., PhD, Professor, Oral Pathology department, Faculty of Dentistry, Misr International University, Egypt
AUTHOR
Wafaa
El.Hossary
wafaaelhosary@gmail.com
4
B.D.S., MSc., PhD, Associate professor, Oral Pathology department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
AUTHOR